foxp3 1 x permeabilisation buffer Search Results


foxp3 1x perm solution  (Thermo Fisher)
86
Thermo Fisher foxp3 1x perm solution
( A-B ) Overview of experimental design. 3’ scRNA-seq <t>(10X</t> Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).
Foxp3 1x Perm Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/foxp3 1x perm solution/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
foxp3 1x perm solution - by Bioz Stars, 2026-03
86/100 stars
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foxp3 1 x permeabilisation buffer  (Thermo Fisher)
90
Thermo Fisher foxp3 1 x permeabilisation buffer
( A-B ) Overview of experimental design. 3’ scRNA-seq <t>(10X</t> Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).
Foxp3 1 X Permeabilisation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/foxp3 1 x permeabilisation buffer/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
foxp3 1 x permeabilisation buffer - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
foxp3 transcription factor fixation permeabilization kit  (Thermo Fisher)
86
Thermo Fisher foxp3 transcription factor fixation permeabilization kit
( A-B ) Overview of experimental design. 3’ scRNA-seq <t>(10X</t> Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).
Foxp3 Transcription Factor Fixation Permeabilization Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/foxp3 transcription factor fixation permeabilization kit/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
foxp3 transcription factor fixation permeabilization kit - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier

Image Search Results


( A-B ) Overview of experimental design. 3’ scRNA-seq (10X Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).

Journal: bioRxiv

Article Title: Single-cell transcriptomic analysis of skeletal muscle regeneration across mouse lifespan identifies altered stem cell states associated with senescence

doi: 10.1101/2023.05.25.542370

Figure Lengend Snippet: ( A-B ) Overview of experimental design. 3’ scRNA-seq (10X Chromium v2 and v3) was performed on dissociated tibialis anterior (TA) muscles from young (4-7 mo), old (20 mo), and geriatric (26 mo) mice (both sexes) 0-7 days post-notexin injury (dpi) with n = 3-4 replicates per age and dpi ( B ). ( C ) Processing workflow. Each scRNA-seq sample was aligned to the mm10 mouse reference genome, ambient RNA was removed by SoupX, low quality cells were identified and removed, and doublets were identified and removed. All samples were then integrated with Harmony, resulting in a final dataset containing 273,923 cells from 65 samples. See Supplementary Figure 1 and Extended Data File 1 for additional detail. ( D ) Fraction of cells from each age group. ( E ) Fraction of cells from each dpi within each age group. ( F - G ) UMAP representations of the final dataset. Cells colored by manually assigned cell type IDs based on the expression of hallmark skeletal muscle genes (see Supplementary and ) ( F ). Cells are colored by age group, with all other cells in gray ( G ).

Article Snippet: After incubating we followed the manufacturer’s protocol (FOXP3 Transcription factor fixation/permeabilization kit, eBioscience # 00-5521-00) and cells were washed with staining buffer, spun, and resuspended in FoxP3 1x perm solution (10x Permeabilization buffer, Invitrogen # 00-8333-56) and incubated for 30 minutes at 4C in the dark.

Techniques: Expressing